OPTIMIZATION OF METHODS FOR THE PRODUCTION AND REFOLDING OF BIOLOGICALLY ACTIVE DISULFIDE BOND-RICH ANTIBODY FRAGMENTS IN MICROBIAL HOSTS

Antibodies have been used for basic research, clinical diagnostics, and therapeutic applications.Escherichia coli is one of the organisms of choice for the production of recombinant antibodies.Variable antibody genes have canonical and non-canonical disulfide bonds that are formed by the oxidation of a pair of click here cysteines.However, the high

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